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SI Materials and Methods - Nucleic Acids Research

For pRFPwt, DNA oligomer pairs (SI Table 1, [#3,#4] and [#5,#6]) were phosphorylated with T4PNK, and annealed. The DNA fragments [3,4], [5,6], ...

http://nar.oxfordjournals.org/content/suppl/2010/08/01/gkq706.DC1/Supporting_Information_7-19-2010.docx

Date added: August 18, 2016 - Views: 1

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RNA::Protein Crosslinking and Immunoprecipitation in vivo ...

RQ Dnase (Promega: 9PIM610), T1 RNAse (Fermentas: EN0541), T4 Polynucleotide Kinase (T4PNK) (Fermentas: ... Protein Crosslinking and Immunoprecipitation in vivo ...

http://www.eurasnet.info/files/files/NetworkofExcellence/Book/Chpt22b_Stamm.doc

Date added: September 20, 2016 - Views: 1

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www.researchgate.net

... and T4PNK to phosphorylate the 5’-OH. For Illumina adapter ligation, a single adenine nucleotide overhang must be added to the polished DNA.

https://www.researchgate.net/profile/Mark_Entman/publication/262191169_Steroid_Receptor_Coactivator-2_Is_a_Dual_Regulator_of_Cardiac_Transcription_Factor_Function/links/0deec53baefdf883f1000000

Date added: January 15, 2017 - Views: 1

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交流】平末端连接构建载体的一些个人经验 - 2006/08/20 21:33

... (请参考t4pnk(点击进入产品介绍)。普通taq酶pcr以后可以补平再做连接,但是仍然需要磷酸化,当然如果是pcr ...

http://api.biodiscover.com/storages/jopphoekppcljlpleljldkdlplgkflckbododocoeohojogoaohoopiojobobofoopelpldl.down

Date added: October 23, 2016 - Views: 1

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4 - img.bio.uni-goettingen.de

... (T4PNK) 75 ng des unmarkierten dT50 und ein entsprechendes Volumen des resuspendierten Dig-dT50 wurden zur Ermittlung der Anzahl der angehängten Dig´-UTP´s ...

http://www.img.bio.uni-goettingen.de/ms-www/internal/methods/DNA/Enzymatische%20Reaktionenan%20DNA.doc

Date added: January 15, 2017 - Views: 1