T4pnk pdfs

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RNaseIII and T4 Polynucleotide Kinase sequence biases and ...

RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library ... T4PNK, Sequence bias, Heat fragmentation, OptiKinase, RNA-seq

https://repositories.lib.utexas.edu/bitstream/handle/2152/27958/1745-6150-8-16.pdf;sequence=1

Date added: August 19, 2016 - Views: 1

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T4 Polynucleotide Kinase - Enzymatics

(additional information on reverse side) Protein Concentration Product Description: T4 Polynucleotide Kinase

http://www.enzymatics.com/pdf/Y904L.pdf

Date added: August 19, 2016 - Views: 1

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T4 Polynucleotide Kinase - lifescience.toyobo.co.jp

T4 Polynucleotide Kinase Code No. PNK-111 Lot No. ***** Storage Store at -20℃ Size 1,500units Source : Escherichia coli JM109 which is carrying T4 pse T gene.

http://lifescience.toyobo.co.jp/user_data/pdf/products/DS_PNK1.pdf

Date added: December 27, 2016 - Views: 1

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T4 Polynucleotide Kinase - Takara Bio Inc

v201206Da Note This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do

http://catalog.takara-bio.co.jp/PDFS/2021S_DS_j.pdf

Date added: August 19, 2016 - Views: 1

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Ben-Shahar Lab CRISPR/RMCE Protocol December 2015

Ben-Shahar Lab CRISPR/RMCE Protocol December 2015 Before you begin ... ii. 2 uL 10x T4PNK buffer iii. 2 uL 10 mM ATP (if not included in buffer, NEB) iv.

http://biology4.wustl.edu/faculty/ben-shahar/Protocols/Ben-Shahar%20Lab%20CRISPR%20Protocol%20Dec%2015.pdf

Date added: August 18, 2016 - Views: 1

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SVPD-post-labeling detection of oxidative damage negates the ...

of adventitious oxidative effects during labeling deems that an optimized SVPD-post-labeling procedure should be ... (T4PNK) and [γ-32P]ATP, ...

http://carcin.oxfordjournals.org/content/20/3/503.full.pdf

Date added: September 4, 2016 - Views: 1

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Molecular basis of ribosome recognition and mRNA hydrolysis ...

Molecular basis of ribosome recognition and mRNA hydrolysis by the E. coliYafQ toxin Tatsuya Maehigashi ... (T4PNK;New EnglandBiolabs)wasincubatedfor6hat37 ...

http://www.biochem.emory.edu/dunham/documents/Maehigashi-NAR-2015.pdf

Date added: August 19, 2016 - Views: 1

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Ben-Shahar CRISPR Protocol 07 2016 - biology4.wustl.edu

ii. 2 uL 10x T4PNK buffer iii. 2 uL 10 mM ATP (if not included in buffer, NEB) iv. 1 uL T4 Polynucleotide Kinase v. 14.5 uL H20 5 vi. 20 uL total vii.

http://biology4.wustl.edu/faculty/ben-shahar/Protocols/Ben-Shahar%20CRISPR%20Protocol%2007_2016%20.pdf

Date added: February 10, 2017 - Views: 1

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Filter-Binding Assays - Home - Springer

Filter-Binding Assays Peter G. Stockley 1. Introduction ... T4PNK reaction buffer, 10X: 0.5M Tris-HCl, pH 7.6, O.lM MgCl,, 0.05M dithiothreitol. 13.

http://link.springer.com/content/pdf/10.1385/0-89603-256-6:251.pdf

Date added: February 10, 2017 - Views: 1

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Molecular characterization of Plasmodium falciparum putative ...

T4PNK(Roche).Double-strandedsubstrateswereannealedbyslow cooling in 70mM Tris–HCl pH 7.6, 10mM MgCl2, 5mM DTT. The 5 -labeled3 - ...

https://www.researchgate.net/profile/Michael_Weinfeld/publication/51551563_Molecular__Biochemical_Parasitology/links/54cbd2110cf24601c0899d6c.pdf?inViewer=true&pdfJsDownload=true&disableCoverPage=true&origin=publication_detail

Date added: February 10, 2017 - Views: 1

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Supplemental Information Crystal Structure of Xanthomonas ...

kinase domain of T4pnk, which are structurally conserved in spite of very low pair wise sequence identity.

http://www.cell.com/cms/attachment/2035785909/2051271654/mmc1.pdf

Date added: September 4, 2016 - Views: 1

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Farnham Lab Puro CRISPR Protocol 20160115

T4PNK ’ 1’µl’ 6.5µl ... Farnham Lab_Puro CRISPR Protocol_20160115 Author: Peggy Farnham Created Date: 1/15/2016 4:35:12 PM ...

http://farnhamlab.com/protocols/Farnham%20Lab_Puro%20CRISPR%20Protocol_20160115.pdf

Date added: August 18, 2016 - Views: 5

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CRISPR&Castarget)site)cloning) 1. sgRNA)design:))

T4PNK! 0.5μl! H20! 6.5μl! Total! 10μl!! PCRProgram:! 37C!45:00min ! 95C! 2:30min! cool!down!at0.1C/s!to!22C! pause!!!!! Heckl!et!al.,!NatureBiotechnology2013!

https://www.addgene.org/static/data/plasmids/57/57826/57826-attachment_n4-jQ6ZzmxPM.pdf

Date added: August 19, 2016 - Views: 1

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Identification of a conserved 5′-dRP lyase activity in ...

TdT,T4PNK,humanAPendonucleaseI(hAPE1),E.coli UracilDNAGlycosylase(UDG)andE.coliEndoIII,were fromNewEnglandBiolabs.Thrombinwasobtainedfrom Novagen.

http://nar.oxfordjournals.org/content/44/4/1833.full.pdf

Date added: November 24, 2016 - Views: 1

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Canonical Wnt signaling is required for development of ...

using T4PNK and ligated into the plasmid SmaI-digested p2lox-empty to generate p2lox.sbcat. After targeting to A2lox ES cells, A2lox.sbcat clones

http://dev.biologists.org/content/develop/133/19/3787.full.pdf

Date added: September 22, 2016 - Views: 1

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Efficient processing of abasic sites by bacterial ...

TdT, T4PNK, hAPE1,EscherichiacoliUracilDNAGlycosylase(UDG), E.coliEndoIII,nickaseNt.BStNBI,T4DNAligaseandre-strictionenzymesAatII,HindIIIandSmaIwereobtained

http://nar.oxfordjournals.org/content/early/2014/10/29/nar.gku1029.full.pdf

Date added: February 10, 2017 - Views: 1

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Recycling of Peptidyl-tRNAs by Peptidyl-tRNA Hydrolase ...

(T4PNK) treatment. Both samples (control and oxidized tRNA) were ligated with the T4PNK-treated Cy3-tagging oligonucleotide under the

http://aac.asm.org/content/57/4/1617.full.pdf?origin=publication_detail

Date added: February 10, 2017 - Views: 1

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Benchmarks - BioTechniques

Benchmarks genomic DNA as template. For exa-m ple, if the ligation took place between the first and the fifthSau3A1 site i n Figure 1, PCR between the primers P1

http://www.biotechniques.com/multimedia/archive/00011/97226bm08_11187a.pdf

Date added: August 19, 2016 - Views: 1

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FUNDAMENTAL GENETIC PROCESSES AND MECHANISMS AN ENZYMATIC ...

fundamental genetic processes and mechanisms 16 an enzymatic synthesis of 5’-end substituted oligonucleotides using t4 polynucleotide kinase and γ-amides of

http://www.bionet.nsc.ru/meeting/bgrs_proceedings/papers/2002/BGRS_2002_4_003.pdf

Date added: August 19, 2016 - Views: 1

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An improved inverse PCR protocol for the generation of T-vectors

nucleotidekinase(T4PNK)fromTaKaRaBiotechnology(Dalian,China), ddTTPfromSigma-Aldrich(StLouis,USA),Taq DNApolymerasewith ThermoPol ...

http://abbs.oxfordjournals.org/content/47/2/142.full.pdf

Date added: August 19, 2016 - Views: 1

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In vitro RNA Cleavage Assay for Argonaute-Family Proteins

In vitro RNA Cleavage Assay for Argonaute-Family Proteins Keita Miyoshi, ... T4 Polynucleotide Kinase (T4PNK) and 10X PNK reaction buffer (TaKaRa, Shiga, Japan).

http://link.springer.com/content/pdf/10.1007/978-1-59745-191-8_3.pdf

Date added: August 19, 2016 - Views: 4

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ATPγS - jenabioscience.com

... (T4PNK)ofDNA[9]and RNA[9,10] Agonisticligand,mainlyfornucleosidereceptorA1 Nucleosidephosphatesstabilizedagainsthydrolyticdegradationcan ...

https://www.jenabioscience.com/images/PDF/NU-406.pdf

Date added: August 19, 2016 - Views: 1

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a novel class-II restriction endonuclease from Micrococcus c ...

T4PNK per pmol 5 ‘-termini in the presence of 50 ,&i [y-‘*P]ATP [ 121. The efficiency of labeling was determined by measuring the rate of incorporation of ...

https://www.researchgate.net/profile/Udo_Reischl/publication/20992839_McrI_a_novel_class-II_restriction_endonuclease_from_Micrococcus_cryophilus_recognizing_5%27-CGRYCG-3%27/links/0c96052cc1bcb49beb000000.pdf

Date added: September 22, 2016 - Views: 1

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Identification of Protein Kinases Dysregulated in CD4 T Cells ...

away, N.J.) by end labeling them with T4PNK (Promega, Madison, Wis.). Each 50- l PCR contained 3 mM MgCl 2,1 Mg-free PCR buffer (Promega), 200 M

http://jvi.asm.org/content/75/23/11298.full.pdf

Date added: September 4, 2016 - Views: 1

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Function and Biogenesis of small RNAs in Dictyostelium discoideum

2.17.1 5’ end analysis using Ap and T4PNK..... 19 . 2.17.2 5’ end analysis using TE and TAP ...

https://ibg.uu.se/digitalAssets/174/174488_1tatikonda-naresh-report.pdf

Date added: December 27, 2016 - Views: 1

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Certificate of Analysis & Product Manual - Gene Link

Certificate of Analysis & Product Manual OligoProber™ cDNA Detection Probes ... 10X T4PNK Reaction Buffer 5 µL [γ-32P] ATP 5 μL of 50 pmol of [γ-32P] ATP/µL

http://www.genelink.com/Literature/ps/OligoProber_COA&M_V3.2.pdf

Date added: December 27, 2016 - Views: 1

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Phosphotriester adducts (PTEs): DNA’s overlooked lesion

requirement for T4PNK. However, several postlabelling strategies have been developed based on the fact that the

http://mutage.oxfordjournals.org/content/25/1/3.full.pdf

Date added: September 22, 2016 - Views: 1

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The Spacious Active Site of a Y-Family DNA Polymerase ...

1 Summary Y-family DNA polymerases lack some of the mechanisms that replicative DNA polymerases employ to ensure fidelity, resulting in higher error rates during ...

http://www.jbc.org/content/early/2004/06/21/jbc.M404332200.full.pdf

Date added: August 19, 2016 - Views: 1

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GenerationofLongRNAChainsinWater - jbc.org

kinase(T4PNK,10units/ l,NewEnglandBiolabs),2 lof10 PNKbufferand0.5 lof[ -32P]ATP,followedbyincubationat 37 °C for 30 min. For gel electrophoresis, 10- l aliquots of the

http://www.jbc.org/content/284/48/33206.full.pdf

Date added: December 27, 2016 - Views: 1

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A Genome-Wide Search for Promoters That Respond to Increased ...

Molecular and Cellular Pathobiology A Genome-Wide Search for Promoters That Respond to Increased MYCN Reveals Both New Oncogenic and Tumor Suppressor MicroRNAs Associated

http://cancerres.aacrjournals.org/content/71/11/3841.full.pdf

Date added: September 22, 2016 - Views: 1

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Intravitreal Delivery of Oligonucleotides by Sterically ...

... (T4pnk; Roche Molecular Biochemicals, Mann-heim, Germany), buffer for T4 polynucleotide kinase (Roche Molecular Biochemicals), and [33P] adenosine triphosphate ...

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.326.3487&rep=rep1&type=pdf

Date added: February 10, 2017 - Views: 1

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Mapping Gene Causing X-Linked Recessive Nephrolithiasis to ...

oftheenzymeT4polynucleotidekinase,T4PNK(BRL,Gaithersburg, MD)ina25-Alvolcontaining50mMTris-HCIpH7.5, 10mMMgCI2, 5 mMdithiothreitol, and 1OACiofy32PATP.PCRamplificationwas

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.429.5042&rep=rep1&type=pdf

Date added: February 10, 2017 - Views: 1

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IDENTIFICATION OF ACTIVITIES INVOLVED IN CAG/CTG REPEAT ...

IDENTIFICATION OF ACTIVITIES INVOLVED IN CAG/CTG REPEAT INSTABILITY CAG/CTG repeat instability is associated with at least 14 neurological disorders,

http://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1835&context=gradschool_diss

Date added: August 19, 2016 - Views: 1

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T7 Transcript Length Determination Using Enzymatic RNA Sequencing

T7 Transcript Length Determination Using Enzymatic RNA Sequencing Andrew Siwkowski 1. Introduction ... T4PNK(10U/(JL) 1 ^L 2 Incubate at 37°C for 1 h

http://link.springer.com/content/pdf/10.1385/0-89603-389-9:91.pdf

Date added: August 19, 2016 - Views: 8

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A genome-wide search for promoters that respond to increased ...

T4 DNA polymerase to fill in 5’ overhangs, Klenow polymerase to remove 3’ overhangs, and T4PNK to phosphorylate the 5’-OH. For Illumina adapter ligation, ...

http://cancerres.aacrjournals.org/content/early/2011/04/14/0008-5472.CAN-10-4391.full.pdf

Date added: August 19, 2016 - Views: 1

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AlteredExonUsageintheJuxtamembraneDomainof ...

AlteredExonUsageintheJuxtamembraneDomainof MouseandHumanRONRegulatesReceptorActivityand SignalingSpecificity* Receivedforpublication,June22,2005,andinrevisedform ...

http://www.personal.psu.edu/nxm2/2005%20Publications/2005-Wei-Hao%20et%20al.pdf

Date added: February 10, 2017 - Views: 1

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Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment ...

... (T4PNK)wasobtained from Fermentas, and [ 32- P]ATP was obtained from Perkin-Elmer. Di-thiothreitol (DTT) and isopropyl- -D-1-thiogalactopyranoside (IPTG) ...

http://jb.asm.org/content/195/24/5516.full.pdf?cited-by=yes&legid=jb;195/24/5516

Date added: February 10, 2017 - Views: 1

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Identification of Novel, Highly Expressed Retroviral MicroRNAs ...

... (T4PNK;NewEnglandBioLabs)at37°Cfor1h. RNAsof 25ntwerecollectedusingCentri-Spin40columns(Princeton Separations)andsequencedwithaTruSeqsmallRNAsamplepreparation ...

http://jvi.asm.org/content/88/9/4679.full.pdf

Date added: August 19, 2016 - Views: 1

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Letter Splicing factor SFRS1 recognizes a functionally ...

Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts ... corporation of 32P is not dependent upon T4PNK, indicating that

http://genome.cshlp.org/content/19/3/381.full.pdf

Date added: September 8, 2016 - Views: 1

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Supporting Information Attachment of NMR invisible solubility ...

Attachment of NMR invisible solubility/stability enhancement tag ... were phosphorylated using T4PNK (TAKARA), annealed, and cloned into pGBMCS using NdeI

http://link.springer.com/content/esm/art:10.1007/s10858-008-9296-5/file/MediaObjects/10858_2008_9296_MOESM1_ESM.pdf

Date added: February 10, 2017 - Views: 1

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Splicing Factor SFRS1 Recognizes a Functionally Diverse ...

Splicing Factor SFRS1 Recognizes a Functionally Diverse Landscape ... upon T4PNK, indicating that this signal is unrelated to SFRS1-RNA complexes (Fig. 1B, lanes

http://genome.cshlp.org/content/early/2008/12/30/gr.082503.108.full.pdf

Date added: August 19, 2016 - Views: 1

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Brief report Microsatellite loci for Parnassius mnemosyne ...

pL T4PNK and 5.0 p1 [Y~~PI-~ATP for 30 min at 37"C, then 90°C for 2 min. The amplification proto- col comprised an initial denaturation at 94°C (3 min) followed ...

https://www.researchgate.net/profile/Emese_MEGLECZ/publication/228039756_Microsatellite_Loci_for_Parnassius_Mnemosyne_Lepidoptera/links/0fcfd50b68012b1649000000.pdf

Date added: November 24, 2016 - Views: 1

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Sequence Complementarity-Driven Nonenzymatic Ligation of RNA

of T4 polynucleotide kinase(T4PNK,10units/μL, New England Biolabs), 2μLof10PNK buffer, and 0.5 μLof [γ-32P]ATP, followed by incubation at 37 Cfor30min.For

https://www.researchgate.net/profile/Giovanna_Costanzo/publication/50248374_Sequence_Complementarity-Driven_Nonenzymatic_Ligation_of_RNA/links/5416e5fe0cf2bb7347db7cb5.pdf?inViewer=0&pdfJsDownload=0&origin=publication_detail

Date added: February 10, 2017 - Views: 1

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Peripheral Blood Mononuclear Cells Express Mutated NCCT mRNA ...

aetin DNA was T4PNK labeled with [32P] yATP toaspecific activity of2.5.tCi/pmol. Thefilterwasprehybridized at42#{176}Cfor2h,

http://jasn.asnjournals.org/content/9/5/819.full.pdf

Date added: December 27, 2016 - Views: 1

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no. pp. 1990 optional E.coli locus encodes latent form of ...

infected with a T4pnk- strain the level of the tRNALYS fragments wasnot augmented (Figure 3) norwasthe level of the remaining intact tRNALyS reduced (not shown). In

https://www.researchgate.net/profile/Gabriel_Kaufmann/publication/21463481_The_optional_E_coli_prr_locus_encodes_a_latent_form_of_phage_T4-induced_anticodon_nuclease/links/0fcfd50f7a602c4e70000000.pdf

Date added: February 10, 2017 - Views: 1

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Bone marrow failure and developmental delay caused by ...

adapter oligonucleotide were 5’end phosphorylated using T4PNK (Thermos Scientific) and ligated to 1 microgram of total RNA at 160C for 48 hours with T4RNA ligase ...

https://www.researchgate.net/profile/Sethu_Gunja/publication/281548801_jmedgenet-2015-103292supp/links/55ed481408aeb6516268d4a2.pdf?inViewer=0&pdfJsDownload=0&origin=publication_detail

Date added: February 10, 2017 - Views: 1

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Analysis of Gene Transcription In Situ: Methodological ...

(T4PNK) and nick translation allowed efficient labeling of DNA, providing probes of high specific activity (19-22). Further refinements in hybridization ...

http://www.tandfonline.com/doi/pdf/10.1179/his.1994.17.3.203

Date added: August 19, 2016 - Views: 1

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This is a pre- or post-print of an article published in ...

oligonucleotide were subjected to a T4 Polynucleotide kinase (T4PNK) treatment. Both samples (control and oxidized tRNA) were ligated with the T4PNK treated Cy3-tagging

http://hzi.openrepository.com/hzi/bitstream/10033/279871/1/G%c3%b6deke%20et%20al_final.pdf

Date added: August 19, 2016 - Views: 1

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RESEARCHARTICLE dRNA-SeqRevealsGenomewideTSSsand ...

(T4PNK)thatcatalyses transfer ofγ-phosphate from32P-ATP. Forthis,40pmol ofoligos weremixedwith 4μl[γ-32P]ATP(10μCi/ml) andphosphorylationtook placebyincubation

https://opus.bibliothek.uni-wuerzburg.de/files/13836/Fan_Ben_PLoS.pdf

Date added: February 10, 2017 - Views: 1