T4pnk pdfs

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T4 Polynucleotide Kinase - Enzymatics

(additional information on reverse side) Protein Concentration Product Description: T4 Polynucleotide Kinase

http://www.enzymatics.com/pdf/Y904L.pdf

Date added: August 19, 2016 - Views: 1

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RNaseIII and T4 Polynucleotide Kinase sequence biases and ...

RNaseIII and T4PNK. Conclusions: Our results suggest that the heat fragmentation removed the RNaseIII sequence bias and significantly reduced the pile-ups and gaps.

https://repositories.lib.utexas.edu/bitstream/handle/2152/27958/1745-6150-8-16.pdf?sequence=1

Date added: December 27, 2016 - Views: 1

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T4 Polynucleotide Kinase - Takara Bio Inc

v201206Da Note This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do

http://catalog.takara-bio.co.jp/PDFS/2021S_DS_j.pdf

Date added: August 19, 2016 - Views: 1

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T4 Polynucleotide Kinase - lifescience.toyobo.co.jp

T4 Polynucleotide Kinase Code No. PNK-111 Lot No. ***** Storage Store at -20℃ Size 1,500units Source : Escherichia coli JM109 which is carrying T4 pse T gene.

http://lifescience.toyobo.co.jp/user_data/pdf/products/DS_PNK1.pdf

Date added: December 27, 2016 - Views: 1

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SVPD-post-labeling detection of oxidative damage negates the ...

of adventitious oxidative effects during labeling deems that an optimized SVPD-post-labeling procedure should be ... (T4PNK) and [γ-32P]ATP, ...

http://carcin.oxfordjournals.org/content/20/3/503.full.pdf

Date added: September 4, 2016 - Views: 1

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Molecular characterization of Plasmodium falciparum putative ...

T4PNK(Roche).Double-strandedsubstrateswereannealedbyslow cooling in 70mM Tris–HCl pH 7.6, 10mM MgCl2, 5mM DTT. The 5 -labeled3 - ...

https://www.researchgate.net/profile/Michael_Weinfeld/publication/51551563_Molecular_characterization_of_Plasmodium_falciparum_putative_polynucleotide_kinasephosphatase/links/54cbd2110cf24601c0899d6c.pdf?origin=publication_list

Date added: September 4, 2016 - Views: 1

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Ben-Shahar Lab CRISPR/RMCE Protocol December 2015

Ben-Shahar Lab CRISPR/RMCE Protocol December 2015 ... • Check whether we have any T4PNK (Polynucleotide kinase) in the lab (usually in one of the blue

http://biology4.wustl.edu/faculty/ben-shahar/Protocols/Ben-Shahar%20Lab%20CRISPR%20Protocol%20Dec%2015.pdf

Date added: August 18, 2016 - Views: 1

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Supplemental Information Crystal Structure of Xanthomonas ...

kinase domain of T4pnk, which are structurally conserved in spite of very low pair wise sequence identity.

http://www.cell.com/cms/attachment/2037870128/2052319506/mmc1.pdf

Date added: December 27, 2016 - Views: 1

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CRISPR&Castarget)site)cloning) 1. sgRNA)design:))

T4PNK! 0.5μl! H20! 6.5μl! Total! 10μl!! PCRProgram:! 37C!45:00min ! 95C! 2:30min! cool!down!at0.1C/s!to!22C! pause!!!!! Heckl!et!al.,!NatureBiotechnology2013!

https://www.addgene.org/static/data/plasmids/57/57826/57826-attachment_n4-jQ6ZzmxPM.pdf

Date added: August 19, 2016 - Views: 1

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Farnham Lab Puro CRISPR Protocol 20160115

T4PNK ’ 1’µl’ 6.5µl ... Farnham Lab_Puro CRISPR Protocol_20160115 Author: Peggy Farnham Created Date: 1/15/2016 4:35:12 PM ...

http://farnhamlab.com/protocols/Farnham%20Lab_Puro%20CRISPR%20Protocol_20160115.pdf

Date added: August 18, 2016 - Views: 5

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FUNDAMENTAL GENETIC PROCESSES AND MECHANISMS AN ENZYMATIC ...

fundamental genetic processes and mechanisms 16 an enzymatic synthesis of 5’-end substituted oligonucleotides using t4 polynucleotide kinase and γ-amides of

http://www.bionet.nsc.ru/meeting/bgrs_proceedings/papers/2002/BGRS_2002_4_003.pdf

Date added: August 19, 2016 - Views: 1

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ATPγS - jenabioscience.com

... (T4PNK)ofDNA[9]and RNA[9,10] Agonisticligand,mainlyfornucleosidereceptorA1 Nucleosidephosphatesstabilizedagainsthydrolyticdegradationcan ...

https://www.jenabioscience.com/images/PDF/NU-406.pdf

Date added: August 19, 2016 - Views: 1

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a novel class-II restriction endonuclease from Micrococcus c ...

T4PNK per pmol 5 ‘-termini in the presence of 50 ,&i [y-‘*P]ATP [ 121. The efficiency of labeling was determined by measuring the rate of incorporation of ...

https://www.researchgate.net/profile/Udo_Reischl/publication/20992839_McrI_a_novel_class-II_restriction_endonuclease_from_Micrococcus_cryophilus_recognizing_5%27-CGRYCG-3%27/links/0c96052cc1bcb49beb000000.pdf?origin=publication_list

Date added: August 19, 2016 - Views: 14

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Molecular basis of ribosome recognition and mRNA hydrolysis ...

Molecular basis of ribosome recognition and mRNA hydrolysis by the E. coliYafQ toxin Tatsuya Maehigashi ... (T4PNK;New EnglandBiolabs)wasincubatedfor6hat37 ...

http://www.biochem.emory.edu/dunham/documents/Maehigashi-NAR-2015.pdf

Date added: August 19, 2016 - Views: 1

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Benchmarks - BioTechniques

Benchmarks genomic DNA as template. For exa-m ple, if the ligation took place between ... rate T4PNK-treated primers or ′- 5 phosphate-modified primers into

http://www.biotechniques.com/multimedia/archive/00011/97226bm08_11187a.pdf

Date added: August 19, 2016 - Views: 1

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DOI: 10.1093/nar/gkg756 Long-distance radical cation ...

Long-distance radical cation reactions in DNA three-way junctions: inter-arm interaction and migration through the junction ... (T4PNK) was purchased from New

http://nar.oxfordjournals.org/content/31/19/5692.full.pdf

Date added: August 19, 2016 - Views: 1

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An improved inverse PCR protocol for the generation of T-vectors

nucleotidekinase(T4PNK)fromTaKaRaBiotechnology(Dalian,China), ddTTPfromSigma-Aldrich(StLouis,USA),Taq DNApolymerasewith ThermoPol ...

http://abbs.oxfordjournals.org/content/47/2/142.full.pdf

Date added: August 19, 2016 - Views: 1

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Certificate of Analysis & Product Manual - Gene Link

Certificate of Analysis & Product Manual OligoProber™ cDNA Detection Probes ... 10X T4PNK Reaction Buffer 5 µL [γ-32P] ATP 5 μL of 50 pmol of [γ-32P] ATP/µL

http://www.genelink.com/Literature/ps/OligoProber_COA&M_V3.2.pdf

Date added: December 27, 2016 - Views: 1

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RESEARCHARTICLE FacileSupermolecularAptamerInhibitorsof L ...

Inc.(Coralville,IA).Materials(FITC-dUTPs, dNTPs,T4PNK,phi29polymerase,T4ligase, andbuffers)used forrollingcircle amplificationwerepurchased fromThermo Scientific (Wal-

https://pdfs.semanticscholar.org/9b9a/51daa368f5c489da375f8606c183029ab6af.pdf

Date added: November 24, 2016 - Views: 1

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Comparisonof7,12-Dimethylbenz(a)anthracene ...

genesis-susceptibleline;T4PNK,T4polynucleotidekinase;PEI,polyethyleneimine. andtheestimatesofrealizedheritability(h2)havealreadybeen published(6).

http://cancerres.aacrjournals.org/content/54/17/4635.full.pdf

Date added: September 22, 2016 - Views: 1

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Tumorigenesis in colorectal tumors from patients with ...

Abstract Tumorigenesis of colorectal cancer in patients with hereditary non-polyposis colorectal cancer (HNPCC) has been postulated to follow a different pathway from

http://link.springer.com/content/pdf/10.1007/s004390050585.pdf

Date added: November 24, 2016 - Views: 1

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In vitro RNA Cleavage Assay for Argonaute-Family Proteins

In vitro RNA Cleavage Assay for Argonaute-Family Proteins Keita Miyoshi, ... T4 Polynucleotide Kinase (T4PNK) and 10X PNK reaction buffer (TaKaRa, Shiga, Japan).

http://link.springer.com/content/pdf/10.1007/978-1-59745-191-8_3.pdf

Date added: August 19, 2016 - Views: 4

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Recycling of Peptidyl-tRNAs by Peptidyl-tRNA Hydrolase ...

Recycling of Peptidyl-tRNAs by Peptidyl-tRNA Hydrolase Counteracts Azithromycin-Mediated Effects on Pseudomonas aeruginosa

http://aac.asm.org/content/57/4/1617.full.pdf

Date added: August 19, 2016 - Views: 1

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Phosphotriester adducts (PTEs): DNA’s overlooked lesion

requirement for T4PNK. However, several postlabelling strategies have been developed based on the fact that the

http://mutage.oxfordjournals.org/content/25/1/3.full.pdf

Date added: September 22, 2016 - Views: 1

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IDENTIFICATION OF ACTIVITIES INVOLVED IN CAG/CTG REPEAT ...

IDENTIFICATION OF ACTIVITIES INVOLVED IN CAG/CTG REPEAT INSTABILITY Nelson Lap Shun Chan ... 2.1.8 32P T4 polynucleotide kinase (T4PNK) 5’ end labeling ...

http://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1835&context=gradschool_diss

Date added: August 19, 2016 - Views: 1

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Identification of Protein Kinases Dysregulated in CD4 T Cells ...

away, N.J.) by end labeling them with T4PNK (Promega, Madison, Wis.). Each 50- l PCR contained 3 mM MgCl 2,1 Mg-free PCR buffer (Promega), 200 M

https://www.researchgate.net/profile/Hsing-Jien_Kung/publication/11663025_Identification_of_Protein_Kinases_Dysregulated_in_CD4_T_Cells_in_Pathogenic_versus_Apathogenic_Simian_Immunodeficiency_Virus_Infection/links/547834cb0cf293e2da285e72.pdf?inViewer=true&pdfJsDownload=true&disableCoverPage=true&origin=publication_detail

Date added: December 27, 2016 - Views: 1

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GenerationofLongRNAChainsinWater - jbc.org

kinase(T4PNK,10units/ l,NewEnglandBiolabs),2 lof10 PNKbufferand0.5 lof[ -32P]ATP,followedbyincubationat 37 °C for 30 min. For gel electrophoresis, 10- l aliquots of the

http://www.jbc.org/content/284/48/33206.full.pdf

Date added: December 27, 2016 - Views: 1

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The Reaction of Water with Guanosine Radical Cation in DNA

The Reaction of Water with Guanosine Radical Cation in DNA Frank O. Onyemauwa and Gary B. Schuster* ... (T4PNK) was obtained from New England Biolabs (Beverly, MA).

http://www.bioone.org/doi/pdf/10.1562/2005-09-07-RA-677

Date added: August 19, 2016 - Views: 1

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Intravitreal Delivery of Oligonucleotides by Sterically ...

... (T4pnk; Roche Molecular Biochemicals, Mann-heim, Germany), buffer for T4 polynucleotide kinase (Roche Molecular Biochemicals), and [33P] adenosine triphosphate ...

https://www.researchgate.net/profile/Elias_Fattal/publication/11584953_Intravitreal_delivery_of_oligonucleotides_by_sterically_stabilized_liposomes/links/0f317536b8a565254f000000.pdf

Date added: December 27, 2016 - Views: 1

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Canonical Wnt signaling is required for development of ...

using T4PNK and ligated into the plasmid SmaI-digested p2lox-empty to generate p2lox.sbcat. After targeting to A2lox ES cells, A2lox.sbcat clones

http://dev.biologists.org/content/develop/133/19/3787.full.pdf

Date added: September 22, 2016 - Views: 1

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Transcriptional inhibition and mutagenesis induced by N ...

andT4polynucleotidekinase(T4PNK)andwerefurther digestedwithSfaNI.Theproductswereseparatedby30% native acrylamide:bisacrylamide (19:1) gel and analyzed

http://nar.oxfordjournals.org/content/43/2/1012.full.pdf

Date added: August 19, 2016 - Views: 1

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Brief report Microsatellite loci for Parnassius mnemosyne ...

pL T4PNK and 5.0 p1 [Y~~PI-~ATP for 30 min at 37"C, then 90°C for 2 min. The amplification proto- col comprised an initial denaturation at 94°C (3 min) followed ...

https://www.researchgate.net/profile/Emese_MEGLECZ/publication/228039756_Microsatellite_Loci_for_Parnassius_Mnemosyne_(Lepidoptera)/links/0fcfd50b68012b1649000000.pdf?inViewer=true&disableCoverPage=true&origin=publication_detail

Date added: December 27, 2016 - Views: 1

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Interactions of NBU1 IntN1 and Orf2x Proteins with Attachment ...

... (T4PNK)wasobtained from Fermentas, and [ 32- P]ATP was obtained from Perkin-Elmer. Di-thiothreitol (DTT) and isopropyl- -D-1-thiogalactopyranoside (IPTG) ...

http://jb.asm.org/content/195/24/5516.full.pdf

Date added: September 22, 2016 - Views: 1

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Version(11/2011(AES( MORL%Solution-BasedTargetedGenomic ...

10U/!μl!T4PNK! % 5μl% % % % 2.!Vortex!and!aliquot!55μl!of!master!mix!intoeachsample!tube!containing!the!beads!and45μl%of%eluate!from!the!

http://www.biomedcentral.com/content/supplementary/1472-6750-12-20-s1.pdf

Date added: August 19, 2016 - Views: 1

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no. pp. 1990 optional E.coli locus encodes latent form of ...

infected with a T4pnk- strain the level of the tRNALYS fragments wasnot augmented (Figure 3) norwasthe level of the remaining intact tRNALyS reduced (not shown). In

https://www.researchgate.net/profile/Gabriel_Kaufmann/publication/21463481_The_optional_E._coli_prr_locus_encodes_a_latent_form_of_phage_T4-induced_anticodon_nuclease/links/0fcfd50f7a602c4e70000000.pdf?disableCoverPage=true

Date added: September 22, 2016 - Views: 1

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DetectionofDNAAdductsinHumanOralTissue ...

DetectionofDNAAdductsinHumanOralTissue:CorrelationofAdductLevels withTobaccoSmokingandDifferentialEnhancementofAdductsUsingthe ... T4PNK,T4polynucleotidekinase: ...

http://cancerres.aacrjournals.org/content/53/7/1522.full.pdf

Date added: August 19, 2016 - Views: 1

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Bone marrow failure and developmental delay caused by ...

adapter oligonucleotide were 5’end phosphorylated using T4PNK (Thermos Scientific) and ligated to 1 microgram of total RNA at 160C for 48 hours with T4RNA ligase ...

http://jmg.bmj.com/content/suppl/2015/09/04/jmedgenet-2015-103292.DC1/jmedgenet-2015-103292supp.pdf

Date added: August 19, 2016 - Views: 1

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Function and Biogenesis of small RNAs in Dictyostelium discoideum

2.17.1 5’ end analysis using Ap and T4PNK..... 19 . 2.17.2 5’ end analysis using TE and TAP ...

https://ibg.uu.se/digitalAssets/174/174488_1tatikonda-naresh-report.pdf

Date added: December 27, 2016 - Views: 1

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AlteredExonUsageintheJuxtamembraneDomainof ...

AlteredExonUsageintheJuxtamembraneDomainof MouseandHumanRONRegulatesReceptorActivityand SignalingSpecificity* Receivedforpublication,June22,2005,andinrevisedform ...

http://www.jbc.org/content/280/48/40241.full.pdf

Date added: September 4, 2016 - Views: 1

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ANewFlavonolGlycosideGallateEsterfrom Acerokamotoanum andIts ...

The anti-HIV-1 integrase activities of the A. okamo-toanumisolateswereinvestigated. AsshowninTable 2,compounds6and1showedstronginhibitoryactivity ... (T4PNK,NewEngland

http://pharmanet.com.br/pdf/np970171q.pdf

Date added: August 19, 2016 - Views: 1

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Peripheral Blood Mononuclear Cells Express Mutated NCCT mRNA ...

aetin DNA was T4PNK labeled with [32P] yATP toaspecific activity of2.5.tCi/pmol. Thefilterwasprehybridized at42#{176}Cfor2h,

http://jasn.asnjournals.org/content/9/5/819.full.pdf

Date added: December 27, 2016 - Views: 1

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Product Specifications. OligoProber™ cDNA Amplification ...

10X T4PNK Reaction Buffer ; 5 µL [γ-32P] ATP : 5 μL of 50 pmol of [γ-32P] ATP/µL . T4 PNK: 2 µL (20 units) Water . up to 50 µl : Total Volume . 50 µL :

http://www.genelink.com/Literature/ps/OligoProber_PS_V2.1.pdf

Date added: August 19, 2016 - Views: 1

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Identification of Novel, Highly Expressed Retroviral MicroRNAs ...

Identification of Novel, Highly Expressed Retroviral MicroRNAs in Cells Infected by Bovine Foamy Virus Adam W. Whisnant,a Timo Kehl, bQiuying Bao, Magdalena ...

http://jvi.asm.org/content/88/9/4679.full.pdf?cited-by=yes&legid=jvi;88/9/4679

Date added: December 27, 2016 - Views: 1

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Gene therapy tools: oligonucleotides and peptides

T4PNK T4 polynucleotide kinase TALE Transcription activator-like effector tcDNA Tricyclo-DNA TFO Triplex-forming oligonucleotide TNA Threose nucleic acid TRAP ...

https://su.diva-portal.org/smash/get/diva2:950969/FULLTEXT01.pdf

Date added: December 27, 2016 - Views: 1

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NOTE TO USERS - citeseerx.ist.psu.edu

T4PNK TCDD TCDF TE8 TEMED TNT TRIS TRIS-HCI tRNA Pg PI PM UV v/v v w/v XRE Quantitative structureactivity relationship Sodium dodecyl sulphate

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.630.3938&rep=rep1&type=pdf

Date added: December 27, 2016 - Views: 1

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Adrenocorticotropin/3′,5′-Cyclic AMP-Mediated Transcription ...

phosphorylated by T4PNK; sequences are as follows (all are times two): SFRE LH D, 5 -TCGACTTCCCTGACCTTGTCTGTGACTTCCCTG-ACCTTGTCTGTGA-3 ; SFRE LH R, 5 -TCGATCACAGACAAGGT-

http://press.endocrine.org/doi/pdf/10.1210/en.2003-1093

Date added: December 27, 2016 - Views: 1

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MOLECULAR BIOLOGY ESSENTIALS - thermofisher.lt

T4PolynucleotideKinase(T4PNK)(10U/L) 500U EK0032 2,500U K1231 CloneJETPCRCloningKit 20rxns K1232 40rxns K1422 RapidDNALigationKit 50rxns K1423 150rxns

http://www.thermofisher.lt/templates/files/tiny_mce/pdf/Marketplace_2015_Molecular_Biology_Essentials.pdf

Date added: August 19, 2016 - Views: 1

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Analysis of Gene Transcription In Situ: Methodological ...

(T4PNK) and nick translation allowed efficient labeling of DNA, providing probes of high specific activity (19-22). Further refinements in hybridization ...

http://www.tandfonline.com/doi/pdf/10.1179/his.1994.17.3.203

Date added: August 19, 2016 - Views: 1

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ADDIS ABABA UNIVERSITY

addis ababa university . school of graduate studies . prevalence of molecular markers associated with sulphadoxine-pyrimethamine resistance in plasmodium

http://etd.aau.edu.et/bitstream/123456789/367/2/Elifaged%20Hailemesikel.pdf

Date added: August 19, 2016 - Views: 1