T4pnk pdfs

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RNaseIII and T4 Polynucleotide Kinase sequence biases and ...

RNaseIII and T4PNK. Conclusions: Our results suggest that the heat fragmentation removed the RNaseIII sequence bias and significantly reduced the pile-ups and gaps.

https://repositories.lib.utexas.edu/bitstream/handle/2152/27958/1745-6150-8-16.pdf?sequence=1

Date added: December 27, 2016 - Views: 1

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T4 Polynucleotide Kinase - Enzymatics

(additional information on reverse side) Protein Concentration Product Description: T4 Polynucleotide Kinase

http://www.enzymatics.com/pdf/Y904L.pdf

Date added: August 19, 2016 - Views: 1

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T4 Polynucleotide Kinase - lifescience.toyobo.co.jp

T4 Polynucleotide Kinase Code No. PNK-111 Lot No. ***** Storage Store at -20℃ Size 1,500units Source : Escherichia coli JM109 which is carrying T4 pse T gene.

http://lifescience.toyobo.co.jp/user_data/pdf/products/DS_PNK1.pdf

Date added: December 27, 2016 - Views: 1

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T4 Polynucleotide Kinase - Takara Bio Inc

v201206Da Note This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do

http://catalog.takara-bio.co.jp/PDFS/2021S_DS_j.pdf

Date added: August 19, 2016 - Views: 1

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Ben-Shahar Lab CRISPR/RMCE Protocol December 2015

Ben-Shahar Lab CRISPR/RMCE Protocol December 2015 Before you begin ... ii. 2 uL 10x T4PNK buffer iii. 2 uL 10 mM ATP (if not included in buffer, NEB) iv.

http://biology4.wustl.edu/faculty/ben-shahar/Protocols/Ben-Shahar%20Lab%20CRISPR%20Protocol%20Dec%2015.pdf

Date added: August 18, 2016 - Views: 1

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SVPD-post-labeling detection of oxidative damage negates the ...

of adventitious oxidative effects during labeling deems that an optimized SVPD-post-labeling procedure should be ... (T4PNK) and [γ-32P]ATP, ...

http://carcin.oxfordjournals.org/content/20/3/503.full.pdf

Date added: September 4, 2016 - Views: 1

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CRISPR&Castarget)site)cloning) 1. sgRNA)design:))

T4PNK! 0.5μl! H20! 6.5μl! Total! 10μl!! PCRProgram:! 37C!45:00min ! 95C! 2:30min! cool!down!at0.1C/s!to!22C! pause!!!!! Heckl!et!al.,!NatureBiotechnology2013!

https://www.addgene.org/static/data/plasmids/57/57818/57818-attachment_oMDeOHpjcyvo.pdf

Date added: March 27, 2017 - Views: 1

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Molecular characterization of Plasmodium falciparum putative ...

T4PNK(Roche).Double-strandedsubstrateswereannealedbyslow cooling in 70mM Tris–HCl pH 7.6, 10mM MgCl2, 5mM DTT. The 5 -labeled3 - ...

https://www.researchgate.net/profile/Michael_Weinfeld/publication/51551563_Molecular_characterization_of_Plasmodium_falciparum_putative_polynucleotide_kinasephosphatase/links/54cbd2110cf24601c0899d6c.pdf

Date added: March 27, 2017 - Views: 1

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Supplemental Information Crystal Structure of Xanthomonas ...

kinase domain of T4pnk, which are structurally conserved in spite of very low pair wise sequence identity.

https://www.researchgate.net/profile/Jiamin_Miao/publication/281746637_mmc1/links/55f6ee1108aeafc8abf51ed7.pdf?origin=publication_list

Date added: November 24, 2016 - Views: 1

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Ben-Shahar CRISPR Protocol 07 2016 - biology4.wustl.edu

ii. 2 uL 10x T4PNK buffer iii. 2 uL 10 mM ATP (if not included in buffer, NEB) iv. 1 uL T4 Polynucleotide Kinase v. 14.5 uL H20 5 vi. 20 uL total vii.

http://biology4.wustl.edu/faculty/ben-shahar/Protocols/Ben-Shahar%20CRISPR%20Protocol%2007_2016%20.pdf

Date added: February 10, 2017 - Views: 1

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Ethylation Interference - Springer

the ethylation interference experiments and by data from a range of ... T4PNK reaction buffer, 10X: OSM Tris-HCl, pH 7.6, O.lM MgC12, 0.05M dithiothreitol.

http://link.springer.com/content/pdf/10.1385/0-89603-256-6:125.pdf

Date added: November 24, 2016 - Views: 1

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Graveley Lab CRISPR Genome Editing followed by RNA-seq ...

T4PNK(NEBM0201S) 0.5 l ddH 2O 6.5 l Totalvolume 10 l 2. Please use the T4 Ligation Buffer since the buffer supplied with the T4 PNKenzymedoesnotincludeATP ...

https://www.encodeproject.org/documents/05ed39ff-5d3b-402c-8d47-a4586d8a2aba/@@download/attachment/HNRNPF_BGKcLV02-9.pdf

Date added: March 27, 2017 - Views: 1

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Molecular basis of ribosome recognition and mRNA hydrolysis ...

Molecular basis of ribosome recognition and mRNA hydrolysis by the E. coliYafQ toxin Tatsuya Maehigashi ... (T4PNK;New EnglandBiolabs)wasincubatedfor6hat37 ...

http://www.biochem.emory.edu/dunham/documents/Maehigashi-NAR-2015.pdf

Date added: August 19, 2016 - Views: 1

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Identification of a conserved 5′-dRP lyase activity in ...

TdT,T4PNK,humanAPendonucleaseI(hAPE1),E.coli UracilDNAGlycosylase(UDG)andE.coliEndoIII,were fromNewEnglandBiolabs.Thrombinwasobtainedfrom Novagen.

http://nar.oxfordjournals.org/content/44/4/1833.full.pdf

Date added: November 24, 2016 - Views: 1

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FUNDAMENTAL GENETIC PROCESSES AND MECHANISMS AN ENZYMATIC ...

fundamental genetic processes and mechanisms 16 an enzymatic synthesis of 5’-end substituted oligonucleotides using t4 polynucleotide kinase and γ-amides of

http://www.bionet.nsc.ru/meeting/bgrs_proceedings/papers/2002/BGRS_2002_4_003.pdf

Date added: August 19, 2016 - Views: 1

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Filter-Binding Assays - Home - Springer

Filter-Binding Assays Peter G. Stockley 1. Introduction ... T4PNK reaction buffer, 10X: 0.5M Tris-HCl, pH 7.6, O.lM MgCl,, 0.05M dithiothreitol. 13.

http://link.springer.com/content/pdf/10.1385/0-89603-256-6:251.pdf

Date added: February 10, 2017 - Views: 1

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Efficient processing of abasic sites by bacterial ...

TdT, T4PNK, hAPE1,EscherichiacoliUracilDNAGlycosylase(UDG), E.coliEndoIII,nickaseNt.BStNBI,T4DNAligaseandre-strictionenzymesAatII,HindIIIandSmaIwereobtained

http://nar.oxfordjournals.org/content/early/2014/10/29/nar.gku1029.full.pdf

Date added: February 10, 2017 - Views: 1

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GenerationofLongRNAChainsinWater - jbc.org

kinase(T4PNK,10units/ l,NewEnglandBiolabs),2 lof10 PNKbufferand0.5 lof[ -32P]ATP,followedbyincubationat 37 °C for 30 min. For gel electrophoresis, 10- l aliquots of the

http://www.jbc.org/content/284/48/33206.full.pdf

Date added: December 27, 2016 - Views: 1

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Expression of short peptides in vivo to modulate protein ...

T4PNK T4 polynucleotide kinase VDR Vitamin D receptor VDRE Vitamin D response element VD Vitamin D VI . Introduction ...

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.427.9803&rep=rep1&type=pdf

Date added: March 27, 2017 - Views: 1

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ATPγS - jenabioscience.com

... (T4PNK)ofDNA[9]and RNA[9,10] Agonisticligand,mainlyfornucleosidereceptorA1 Nucleosidephosphatesstabilizedagainsthydrolyticdegradationcan ...

https://www.jenabioscience.com/images/PDF/NU-406.pdf

Date added: August 19, 2016 - Views: 1

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Benchmarks - BioTechniques

Benchmarks genomic DNA as template. For exa-m ple, if the ligation took place between ... rate T4PNK-treated primers or ′- 5 phosphate-modified primers into

http://www.biotechniques.com/multimedia/archive/00011/97226bm08_11187a.pdf

Date added: August 19, 2016 - Views: 1

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Farnham Lab Puro CRISPR Protocol 20160115

T4PNK ’ 1’µl’ 6.5µl ... Farnham Lab_Puro CRISPR Protocol_20160115 Author: Peggy Farnham Created Date: 1/15/2016 4:35:12 PM ...

http://farnhamlab.com/protocols/Farnham%20Lab_Puro%20CRISPR%20Protocol_20160115.pdf

Date added: August 18, 2016 - Views: 5

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Phosphotriester adducts (PTEs): DNA’s overlooked lesion

requirement for T4PNK. However, several postlabelling strategies have been developed based on the fact that the

http://mutage.oxfordjournals.org/content/25/1/3.full.pdf

Date added: September 22, 2016 - Views: 1

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RESEARCHARTICLE FacileSupermolecularAptamerInhibitorsof L ...

Inc.(Coralville,IA).Materials(FITC-dUTPs, dNTPs,T4PNK,phi29polymerase,T4ligase, andbuffers)used forrollingcircle amplificationwerepurchased fromThermo Scientific (Wal-

https://www.researchgate.net/profile/Monsur_Ali/publication/274382621_Facile_supermolecular_aptamer_inhibitors_of_L-selectin/links/551d75b30cf213ef063e593a.pdf?disableCoverPage=true

Date added: March 27, 2017 - Views: 1

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IDENTIFICATION OF ACTIVITIES INVOLVED IN CAG/CTG REPEAT ...

IDENTIFICATION OF ACTIVITIES INVOLVED IN CAG/CTG REPEAT INSTABILITY Nelson Lap Shun Chan ... 2.1.8 32P T4 polynucleotide kinase (T4PNK) 5’ end labeling ...

http://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1835&context=gradschool_diss

Date added: August 19, 2016 - Views: 1

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Certificate of Analysis & Product Manual

Certificate of Analysis & Product Manual OligoProber™ cDNA Detection Probes ... 10X T4PNK Reaction Buffer 5 µL [γ-32P] ATP 5 μL of 50 pmol of [γ-32P] ATP/µL

https://www.genelink.com/Literature/ps/OligoProber_COA&M_V3.2.pdf

Date added: August 19, 2016 - Views: 1

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a novel class-II restriction endonuclease from Micrococcus c ...

T4PNK per pmol 5 ‘-termini in the presence of 50 ,&i [y-‘*P]ATP [ 121. The efficiency of labeling was determined by measuring the rate of incorporation of ...

https://www.researchgate.net/profile/Udo_Reischl/publication/20992839_McrI_a_novel_class-II_restriction_endonuclease_from_Micrococcus_cryophilus_recognizing_5%27-CGRYCG-3%27/links/0c96052cc1bcb49beb000000.pdf?origin=publication_detail

Date added: March 27, 2017 - Views: 1

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Canonical Wnt signaling is required for development of ...

using T4PNK and ligated into the plasmid SmaI-digested p2lox-empty to generate p2lox.sbcat. After targeting to A2lox ES cells, A2lox.sbcat clones

http://dev.biologists.org/content/develop/133/19/3787.full.pdf

Date added: September 22, 2016 - Views: 1

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A Genome-Wide Search for Promoters That Respond to Increased ...

Molecular and Cellular Pathobiology A Genome-Wide Search for Promoters That Respond to Increased MYCN Reveals Both New Oncogenic and Tumor Suppressor MicroRNAs Associated

http://cancerres.aacrjournals.org/content/71/11/3841.full.pdf

Date added: September 22, 2016 - Views: 1

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Recycling of Peptidyl-tRNAs by Peptidyl-tRNA Hydrolase ...

(T4PNK) treatment. Both samples (control and oxidized tRNA) were ligated with the T4PNK-treated Cy3-tagging oligonucleotide under the

http://aac.asm.org/content/57/4/1617.full.pdf?origin=publication_detail

Date added: February 10, 2017 - Views: 1

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Comparison of the Mitochondrial Genomes and Steady State ...

RESEARCHARTICLE ComparisonoftheMitochondrialGenomes andSteadyStateTranscriptomesofTwo StrainsoftheTrypanosomatidParasite, Leishmaniatarentolae LarrySimpson1*,StephenM ...

http://dna.kdna.ucla.edu/simpsonlab/Lab%20publications/plos%20paper1.pdf

Date added: March 27, 2017 - Views: 1

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Ren mRNA-seq protocols v090113 - encodeproject.org

T4PNK! 1!! 9.7! Add9.7µLof!theER!reaction!mixto!thetubewith!the50 µLsheared!cDNA!and!mixcarefully.!! Incubate30minat20oCinThermomixer.!!

https://www.encodeproject.org/documents/0a7c7860-4801-456d-baaa-0f3ac6d99aa7/@@download/attachment/Ren_mRNA-seq_protocols_v090113.pdf

Date added: September 4, 2016 - Views: 1

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The Spacious Active Site of a Y-Family DNA Polymerase ...

1 Summary Y-family DNA polymerases lack some of the mechanisms that replicative DNA polymerases employ to ensure fidelity, resulting in higher error rates during ...

http://www.jbc.org/content/early/2004/06/21/jbc.M404332200.full.pdf

Date added: August 19, 2016 - Views: 1