Cosmids Ppt ppts

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 28, 2016 - Views: 5

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Vectors - College of Computer, Mathematical, and Natural Sciences

Most vectors are genetically engineered plasmids or phages. There are also cosmid vectors, ... Cosmids combine essential elements of a plasmid and Lambda systems.

http://www.clfs.umd.edu/grad/mlfsc/res/RecombomamtDNA&Medicine.ppt

Date added: August 22, 2016 - Views: 1

ppt
PowerPoint Presentation

... Cosmids PACs BACs Bacteriophage Vectors Lambda: Each clone can accept inserts of ~15-20 kb. Replace part of l DNA that is not needed for replication with ...

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: August 27, 2016 - Views: 1

ppt
PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic ...

http://www.pascack.org/cms/lib5/NJ01000238/Centricity/Domain/142/WSSP06-Ch2-LIbraries.ppt

Date added: February 4, 2017 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: August 18, 2016 - Views: 8

ppt
PowerPoint Presentation

... cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: August 20, 2016 - Views: 2

ppt
No Slide Title

... replicatioin from two different organisms Can be replicated in both Often used to shuttle plasmids from bacteria to Cosmids – related to bacteriophages ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: August 27, 2016 - Views: 6

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: August 19, 2016 - Views: 2

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. Brief Chapter Outline I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: August 18, 2016 - Views: 8

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Die Achterbahn - Swansea University

... in E. coli Recombinant cosmid-DNA can easily be confirmed by PCR, restriction analysis and/or sequencing Mutagenised cosmids can be mobilised by conjugation, ...

http://www.swansea.ac.uk/media/media,35188,en.ppt

Date added: August 30, 2016 - Views: 1

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justc/courses/RecombDNA/Week6. ppt - Portland State University

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, deleting insert DNA (to reduce drag on cells) ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: August 19, 2016 - Views: 1

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Foundations of Biology - Geoscience Research Institute

Foundations of Biology ... Drosophila Genome Chromosome Morphology Chromosome Morphology Naming Chromosome Parts Drosophila X Chromosome 2b Cosmids An ...

http://grisda.org/resources/Power_Points/Molecular_Genetics/Fly_Genome.ppt

Date added: August 18, 2016 - Views: 2

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DUAL TRAFFICKING PATHWAYS OF CONNEXINS TO GAP JUNCTIONS

... Progress to date: 105 of 319 cosmids fully processed 11493 independent insertions 10459 insertions in 2520 orfs (of 7825 in total) 4.2 ...

http://www.swansea.ac.uk/media/media,35187,en.ppt

Date added: August 21, 2016 - Views: 1

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Presentazione standard di PowerPoint - Unife

Cloning in Cosmids. Production of overlapping restriction fragments by partial digestion of human genomic DNA with Sau3A. CHROMOSOME WALKING. CHROMOSOME WALKING.

http://m.docente.unife.it/dario.balestra/tecnologie-ricombinanti-2015/5-genoteche-cdnateche.pptx

Date added: August 21, 2016 - Views: 4

ppt
No Slide Title

Cosmids combination of lambda phage and plasmid hold inserts 35-45 kb FIGURE 20.6 3. Artificial Chromosomes YAC (yeast artificial Chromosomes - 500 ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: August 20, 2016 - Views: 5

ppt
PowerPoint Presentation

Fosmids offer higher stability than comparable high copy number cosmids. Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures/chapter_08.ppt

Date added: August 30, 2016 - Views: 1

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HOW TO PREPARE VECTORS TO CARRY YOUR FAVORITE GENE.

Biology 417 Week 1, Lecture #2 With input from: Yung Huang, Luis Sanchez, Lee Lin, Leticia Argueta, Kay Nguyen PGM 2000 Revised 2012 - SBS

http://www.calstatela.edu/faculty/ssharp/slides/%21PGM2012%21/12BiotechRE2012.ppt

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

Cosmids combine phage vectors’ ability to be packaged with the DNA propagation and purification properties of plasmids. Their maximum insert size is between 35 ...

http://forest.mtu.edu/faculty/joshi/new/BL3300%202006/BL3300%202006%20slides/chapter%203/03%20091904%20cpj.ppt

Date added: October 21, 2016 - Views: 1

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Chapter 12

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: August 30, 2016 - Views: 1

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emboj.embopress.org

... (bottom) performed with fluorescent probes derived from recombinant cosmids 130, 1338, 488, 323 and 183 of chromosome XV (Tettelin et al, 1998).

http://emboj.embopress.org/content/23/1/234/F2.ppt

Date added: February 4, 2017 - Views: 1

ppt
PowerPoint Presentation

Title: PowerPoint Presentation Author: Wenun Last modified by: Wenun Created Date: 8/26/2004 6:56:18 AM Document presentation format: On-screen Show

http://mis.agri.cmu.ac.th/download/course/lec_359401_03%BE%D1%B9%B8%D8%C7%D4%C8%C7%A1%C3%C3%C1%C8%D2%CA%B5%C3%EC.ppt

Date added: August 29, 2016 - Views: 1

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บทที่ 18 พันธุศาสตร์และเทคโนโลยีทางดีเอ็นเอ (Genetics and DNA ...

... Cloning by cosmids. The cosmid is cut at a BglII site next to the cos site. Donor genomic DNA is cut using Sau3A, which gives sticky ends compatible with BglII.

https://scibioblog.files.wordpress.com/2011/08/6-gene-tech.ppt

Date added: September 3, 2016 - Views: 1

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... cell-cell spread suppression of class I MHC promoter pA pack HSV-ori bacterial plasmid seq cloning site defective virus cosmids ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: August 30, 2016 - Views: 3

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DNA cloning intro.ppt - Biology

BACTERIOPHAGE LAMBDA COSMID VECTOR Purpose: 1. Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: August 23, 2016 - Views: 9

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Plasmids and Vectors - Molecular Biology for Secondary ...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

... normally not pathogenic, and inexpensive * * * * * * * Figure 3.5 * * * Figure 3.5 Cosmids Contain cos ends of lambda DNA, a plasmid origin of replication, ...

http://www.austincc.edu/awheeler/Files/BITC%201311%20Spring%202009/BITC%201311/chapter%203.ppt

Date added: January 9, 2017 - Views: 1

ppt
PowerPoint Presentation

Title: PowerPoint Presentation Author: Mike Bartlett Last modified by: Justin Courcelle Created Date: 3/31/2003 6:09:42 AM Document presentation format

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: August 22, 2016 - Views: 1

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Lambda Vectors and their replication - Biology

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

Insert size range < 15kb. Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: August 22, 2016 - Views: 2

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Information Encoding in Biological Molecules: DNA and protein ...

... (gamma-PPT-A) gene, partial cds. ... extracted from Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, ...

http://bioinformatics.ca/dtt2004-lectures-day2-22sercbwppt

Date added: August 25, 2016 - Views: 11

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Forward Genetics - University of Colorado Boulder

Forward Genetics Survey ... mor-2 sup-23 evl-7 Genetic map map unit Cloned genes Non-cloned genes mDf4 nDf41 rme-2 unc-5 fem-1 skn-1 Physical map cosmids YACs ...

http://www.colorado.edu/MCDB/HanLab/part2.baisc_forward_genetics.ppt

Date added: August 23, 2016 - Views: 1

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www.cell.com

The cosmids from within the IGLL locus that are used for FISH are indicated on the top line over the region shaded in light red.

http://www.cell.com/ajhg/ppt/S0002-9297(07)62073-4.ppt

Date added: August 30, 2016 - Views: 1