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3 Basic Principles of Recombinant DNA Technology

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3 Basic Principles of Recombinant DNA Technology

3 Basic Principles of Recombinant DNA Technology. ... Cosmids. B. Vectors for Other Organisms. ... Engineered Ti plasmids lack the tumor-causing genes, ...

http://users.ipfw.edu/Mourad/Courses/BIOL%20509/509%202010%20Ch%203.pptx

Date added: August 18, 2016 - Views: 7

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Cloning into Plasmids - Buffalo State College

Cloning into Plasmids Restriction Fragment Cloning & PCR Cloning by the Topo TA™ Method Cloning Vectors The molecular analysis of DNA has been made possible by the ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: August 19, 2016 - Views: 2

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No Slide Title

Molecular Cloning: Construction of a recombinant DNA Molecular Cloning: Cloning Vectors Plasmids Molecular Cloning: Cloning Vectors Plasmids Origin of replication ...

http://www.siumed.edu/~bbartholomew/Lectures/Molecular%20Cloning%2006.ppt

Date added: August 27, 2016 - Views: 2

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Slide 1

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/ProcedureOfGene%20cloning19.21-4-2016/2%20Cloning%20vectors.pptx

Date added: September 7, 2016 - Views: 1

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Plasmid DNA isolation - Assiut University

Cosmids. yeast artificial chromosome. ... Features of many modern Plasmids. Plasmid vectors are used to clone DNA ranging in size from several base pairs to several ...

http://www.aun.edu.eg/molecular_biology/Procedure%20from%20gene%20to%20protein/2%20Cloning%20vectors.pptx

Date added: September 12, 2016 - Views: 1

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Cloning - Sam Houston State University

Plasmids and other types of cloning vectors were used by Human Genome Project ... Cosmids are artificially constructed cloning vectors that carry up to 45 kb of ...

http://www.shsu.edu/~agr_www/documents/CloningPart1PP.pptx

Date added: August 18, 2016 - Views: 2

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PowerPoint Presentation

Lambda Bacteriophage Vectors cos cos cos Selection: Only recombinant phage produce plaques. 1. Size ... Cosmids: Plasmids that contain l cos sites.

http://faculty.smu.edu/borr/ClassNotes/10-17-05.ppt

Date added: August 27, 2016 - Views: 1

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HOW TO PREPARE VECTORS TO CARRY YOUR FAVORITE GENE.

Biology 417 Week 1, Lecture #2 With input from: Yung Huang, Luis Sanchez, Lee Lin, Leticia Argueta, Kay Nguyen PGM 2000 Revised 2012 - SBS

http://www.calstatela.edu/faculty/ssharp/slides/%21PGM2012%21/12BiotechRE2012.ppt

Date added: August 30, 2016 - Views: 1

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Inquiry into Life Twelfth Edition - Regis University

vectors: Plasmids; Phages . ... Cosmids. designed to clone large DNA fragments. Behave as plasmid and phage. ... Inquiry into Life Twelfth Edition

http://academic.regis.edu/jbetz/BL426Lectures-Notes/chapt04%202011.pps

Date added: August 21, 2016 - Views: 2

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PowerPoint Presentation

Cosmids: Plasmids that contain l cos sites. This allows them to be packaged into l particles. But don’t have l genes, therefore can clone larger insert fragments ...

http://faculty.smu.edu/borr/ClassNotes/10-19-05.ppt

Date added: August 28, 2016 - Views: 2

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What is gene clonning? - FaPGenT

One of the first vectors to be developed was pBR322. ... (there are also plasmids called pBR325, ... as cosmids lack all the λgenes and so do not produce plaques.

http://www.fapgent.org/uploads/1/8/9/7/18975273/cpt_6_cloning_vectors_for_e.coli.pptx

Date added: October 22, 2016 - Views: 1

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Biotechnology and Genetic Engineering-PBIO 450/550

... but it can also be 125I Animations for two related uses of expression vectors ... vectors including plasmids, bacteriophage lambda (l), and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: August 18, 2016 - Views: 5

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No Slide Title

Plasmids Cloning Vectors Plasmid Vector Other vectors Shuttle vectors Selection for E coli which contain ... Cosmids combination of lambda phage and plasmid hold ...

http://faculty.mc3.edu/lrehfuss/bit220/ch20.ppt

Date added: August 20, 2016 - Views: 3

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Biotechnology and Genetic Engineering-PBIO 450/550

Biotechnology and Genetic Engineering PBIO 4500 ... How to make and screen cDNA libraries Several different cloning vectors including plasmids, ... and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO4500%20Lecture%203.ppt

Date added: August 27, 2016 - Views: 2

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Viral Vectors - University of Saskatchewan

Viral Vectors May 8 Viral vectors ... plasmid seq cloning site defective virus cosmids HSV genes BACS HSV genes linear plasmid joined end ... gene of interest ...

http://homepage.usask.ca/~vim458/vtmc831/May8.ppt

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

Vectors - EM of Small Plasmid DNA Plasmids are vectors, molecular tools for carrying DNA of interest. Other vectors include bacteriophage, cosmids, ...

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: August 20, 2016 - Views: 2

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Slide 1

While those with hybrid vectors form white colonies. Figure 19-6 A diagram of the plasmid pUC18 showing the polylinker ... Cosmids – behaving as both plasmids and ...

https://aliazamani.files.wordpress.com/2015/07/lec-4-gene-cloning-cont.pptx

Date added: August 27, 2016 - Views: 1

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PowerPoint Template - giyan77.files.wordpress.com

Application of Gene Cloning. Identification of genetic diseases. Putting new genes into animals and plants. Identification of individuals based on their genes

https://giyan77.files.wordpress.com/2014/11/kuliah-ke-7-cloning-dna-up.pptx

Date added: November 28, 2016 - Views: 1

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Competent Cells formation and transformation of competent ...

competent cells formation and transformation of competent cells ... Cosmids: an extra chromosomal circular DNA molecule that combines features of plasmids and ...

http://fac.ksu.edu.sa/sites/default/files/2-competent_cells_formation_and_transformation_of_competent_cells_with_dna_0.pptx

Date added: August 30, 2016 - Views: 1

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Plasmids and Vectors - Molecular Biology for Secondary ...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening ...

https://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: August 30, 2016 - Views: 1

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Title goes in here - cmb-file.persiangig.com

Phage vectors (λ) Cosmids. ... Secretion Vectors. Formation of Inclusion Bodies. May 26, 2013. ... (shuttle vectors) Yeast Integrative Plasmids (YIp)

http://cmb-file.persiangig.com/other/Microbial%20Biotechnology.ppsx

Date added: September 24, 2016 - Views: 1

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PowerPoint Presentation

Molecular Basis for Relationship between Genotype and Phenotype DNA genotype DNA sequence transcription RNA translation amino acid sequence protein function

http://public.gettysburg.edu/~hiraizum/bio211/0909.ppt

Date added: October 21, 2016 - Views: 1

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Lambda Vectors and their replication - Biology

Lambda vectors and their replication Sonita Gafary Biochem 72020 Lambda was first discovered at the Pasteur Institute by Andre Lwoff when he observed strains of E. Coli.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: August 30, 2016 - Views: 1

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Slide 1

Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid

http://ecourse.sl.on.ca/mjsibbald/mjsibbald/08mb/0807mbviral%20vectors%20and%20other%20vectors.ppt

Date added: August 30, 2016 - Views: 1

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Vectors - | College of Computer, Mathematical, and Natural ...

Cosmids are extracted from ... rDNA Objectives Steps in rDNA Processing Genetic Engineering Advances The use of rDNA to Produce Human Insulin Cloning Vectors Plasmids ...

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: September 6, 2016 - Views: 1

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DNA cloning intro.ppt - Biology

PLASMIDS PLASMID VECTORS Plasmid vectors are ≈1.2–3kb and contain: ... size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: August 23, 2016 - Views: 7

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Chapter 4 Molecular Cloning Methods - Xiamen University

Chapter 4 Molecular Cloning Methods ... Endonucleases Vectors Plasmids as Vectors Phages as Vectors λ Phage Vectors Cosmids M13 phage vectors ...

http://yjsy.xmu.edu.cn/Cn/uploads/Chapter%204.ppt

Date added: August 30, 2016 - Views: 1

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Chapter 12

... General PowerPoint Presentation Types of Vectors and DNA delivery systems Types of Vectors Plasmids Plasmid vectors ... vectors Phage Insertion Cosmids ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: August 30, 2016 - Views: 1

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Setting up a transformation--how will the competent cells be ...

Setting up a transformation--how will the competent cells be treated? No plasmid (negative control, nothing should grow on this plate)

http://web.pdx.edu/~justc/courses/RecombDNA/Week5.ppt

Date added: August 30, 2016 - Views: 1

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Vectors for Even Larger Genomic DNA Inserts

General Microbiology (Micr300) Lecture 11 Biotechnology (Text Chapters: 10.15-10.17; 31.1-31.10)

http://instructional1.calstatela.edu/hxu3/Micr300/S06micr300Lec11final.ppt

Date added: November 8, 2016 - Views: 3

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A DNS tulajdonságai hogyan használhatók a manipulációban?

A DNS tulajdonságai hogyan használhatók a manipulációban? Könnyen izolálható, elektroforézis, Összeilleszthető Megolvasztható, kelálható festékekkel ...

http://biokemia.elte.hu/attachments/download/course/33/23

Date added: August 21, 2016 - Views: 1

ppt
PowerPoint Presentation

Plasmid vectors engineered from bacterial plasmids for use in cloning. ... Contain other features similar to plasmids/cosmids such as origin sequence and polylinker.

http://www.bio.miami.edu/mccracken/genetics/lectures/chapter_08.ppt

Date added: August 30, 2016 - Views: 1

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Enzyme Mechanisms - Illinois Institute of Technology

... Shuttle vectors These are plasmids that can operate in two ... Enzyme Mechanisms Author: ... cloning Use of bacteriophage lambda Cosmids ...

http://csrri.iit.edu/~howard/biochem/lecppt/mobio_methods.ppt

Date added: August 18, 2016 - Views: 1

ppt
Slide 1

... Vectors for cloning large piece of DNA Bacteriophage-l and other phage vectors Cosmids ... Phagemid Vectors Contain components from phage chromosomes and plasmids.

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_3.ppt

Date added: August 30, 2016 - Views: 1

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Genetic Engineering - University of North Carolina at Wilmington

Genetic Engineering ... Vector Stable Packaging Cosmids Other Vectors Expression Vectors Secretion ... Splice into Cloning Vehicle Plasmids Viruses Use ...

p://people.uncw.edu/sizemorer

Date added: August 18, 2016 - Views: 4

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Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

Larger DNA Fragments Can Be Cloned in Cosmids and Other Vectors Both λ phage vectors and the more ... Molecular Cloning I: Restriction Enzymes, PCR, Plasmids, and ...

http://www.sut.ac.th/iat/biotech/MontaropSite/Powerpoint/304512_2551.ppt

Date added: August 23, 2016 - Views: 2

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Recombinant DNA Technology for the non-science major.

Recombinant DNA Technology for the non-science major. ... Plasmids 2. Bacteriophages 3. Cosmids ... Most vectors are derived from: 1. Plasmids 2.

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: August 30, 2016 - Views: 1

ppt
Slide 1

Why Plasmids are Good Cloning Vectors small size ... Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites.

http://mb206.wikispaces.com/file/view/MB206_fhs_int_008b_AT_Jan09.ppt

Date added: August 30, 2016 - Views: 1

ppt
Inquiry into Life Twelfth Edition - McGraw Hill Education

... Phagemids are also vectors Like cosmids have aspects of ... classes of vectors: Plasmids Phages Plasmids ... Inquiry into Life Twelfth Edition Author:

http://highered.mheducation.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: August 30, 2016 - Views: 1

ppt
Slide 1

... insertional vectors and replacement vectors Slide 14 Cosmids are plasmids that can be packaged into bacteriophage lamda particles Slide 16 Slide 17 Slide ...

http://getenotes.weebly.com/uploads/1/4/1/9/14194145/genetic_engineering__unit_i__lecture_2.ppt

Date added: August 30, 2016 - Views: 1

ppt
Slide 1

Lecture 14 – Gene Cloning Based on Chapter 08 - Genomics: The Mapping and Sequencing of Genomes Copyright © 2010 Pearson Education Inc.

http://www.auburn.edu/academic/classes/biol/3020/lecture_ppts/L14_DNA%20Cloning_14F.ppt

Date added: August 30, 2016 - Views: 1

ppt
PowerPoint Presentation

... specialized plasmids Vectors containing ... vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and ... Document presentation format ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: August 22, 2016 - Views: 1

ppt
PowerPoint Presentation

... for large-scale sequencing Good compromise between insert size and ease of use Growth and isolation similar to that for plasmids ... vectors are cosmids, ...

http://forest.mtu.edu/faculty/joshi/new/BL3300%202006/BL3300%202006%20slides/chapter%203/03%20091904%20cpj.ppt

Date added: October 21, 2016 - Views: 1

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Enzyme Mechanisms - Illinois Institute of Technology

... Shuttle vectors These are plasmids that can operate in ... inserts up to 40 kbp in size Cosmids in action (fig. 12.9) Shuttle vectors These are plasmids that ...

http://csrri.iit.edu/~howard/biochem/lecppt/recombinantF10_401.ppt

Date added: August 18, 2016 - Views: 1

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Inquiry into Life Twelfth Edition - highered.mheducation.com

Inquiry into Life Twelfth Edition ... Vectors Plasmids As ... via Plaque Hybridization Cosmids M13 Phage Vectors M13 Cloning to Recover ...

http://highered.mheducation.com/sites/dl/free/0073525324/891545/Chapter_04_lecture.ppt

Date added: August 30, 2016 - Views: 1

ppt
Slide 1

The original plate is used to pick bacterial cells with recombinant plasmids that hybridized ... Plasmids Phages Cosmids New generation vectors: Bacterial ...

http://higheredbcs.wiley.com/legacy/college/allison/1118059816/ppt/ch08.ppt

Date added: August 20, 2016 - Views: 1

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DNA Libraries - Western Washington University

... plasmids BACS YACS Transduction (via virus mechanisms); ... (via virus mechanisms); phage cosmids Cloning Step 3 ...vectors contain selectable markers, ...

http://fire.biol.wwu.edu/young/321/LG8.ppt

Date added: October 9, 2016 - Views: 1

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Table of Contents - Midwestern State University

Recombinant DNA and Biotechnology

http://faculty.mwsu.edu/biology/jon.scales/Courses/POB/Lecture_Notes/recDNA05.ppt

Date added: August 30, 2016 - Views: 1

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Biotechnology: Principles, Applications, and Social Implications

Biotechnology Gene therapy, Cloning Heredity No 439 by RNDr. Hana Zoubková, PhD

http://nas.lf3.cuni.cz/materialy/CVSE1M0001/439%20biotechnology%20ang%20zoub(539aeb298ff4d).ppt

Date added: August 20, 2016 - Views: 1